bapta am Search Results


bapta am  (Dojindo Labs)
93
Dojindo Labs bapta am
Bapta Am, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bapta+am/pm11348889-25-11-15?v=Dojindo+Labs
Average 93 stars, based on 1 article reviews
bapta am - by Bioz Stars, 2026-08
93/100 stars
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tetraacetoxymethyl ester bapta am  (Tocris)
95
Tocris tetraacetoxymethyl ester bapta am
Tetraacetoxymethyl Ester Bapta Am, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bapta+am/pmc03650390-130-22-63?v=Tocris
Average 95 stars, based on 1 article reviews
tetraacetoxymethyl ester bapta am - by Bioz Stars, 2026-08
95/100 stars
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6 8 bid  (Tocris)
95
Tocris 6 8 bid
6 8 Bid, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bapta+am/pmc07793167__NIHMS1649676___supplement___5-289-187-189?v=Tocris
Average 95 stars, based on 1 article reviews
6 8 bid - by Bioz Stars, 2026-08
95/100 stars
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bapta am  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology bapta am
Bapta Am, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bapta+am/pmc08967991-213-14-16?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
bapta am - by Bioz Stars, 2026-08
93/100 stars
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bapta am  (Selleck Chemicals)
94
Selleck Chemicals bapta am
HPF induces Dlat-mediated Ca 2+ release to activate Camkkβ-AMPK signaling and adipose thermogenesis. (A) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with Pim/AKK1-IN-1 (1 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (B) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with STO-609 (10 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (C) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Camkkβ siRNA for 24 h and followed by HPF (5 μM) treatment for 24 h. (D) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated <t>with</t> <t>BAPTA-AM</t> (20 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (E) Ca 2+ signal upon HPF (20 μM) stimulation in C3H10T1/2-derived adipocytes transfected with Dlat siRNA ( n = 4). (F) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by STO-609 (10 μM) treatment for 24 h. (G) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h. (H) mRNA levels of the thermogenic genes in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h ( n = 3). (I) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by BAPTA-AM (20 μM) treatment for 24 h. (J) Representative images of JC-1 staining in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h, scale bar = 50 μm. (K) The ratio of JC-1 fluorescence intensity (green to red) in (J) ( n = 6). (L) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with vector or Dlat-Flag plasmids for 48 h, scale bar = 50 μm. (M) Quantification of (L). (N) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with the negative control or Dlat siRNA for 48 h, scale bar = 50 μm. (O) Quantification of (N). Data are presented as mean ± SEM, and tested for normality using Shapiro-Wilk normality test and Kolmogorov-Smirnov test. Significance was assessed by two-way ANOVA (E), or one-way ANOVA (H and K), or Student’s t test (M and O). * P < 0.05; ** P < 0.01; *** P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Bapta Am, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bapta+am/pmc12789730-72-56-57?v=Selleck+Chemicals
Average 94 stars, based on 1 article reviews
bapta am - by Bioz Stars, 2026-08
94/100 stars
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bapta-am  (Cayman Chemical)
90
Cayman Chemical bapta-am
HPF induces Dlat-mediated Ca 2+ release to activate Camkkβ-AMPK signaling and adipose thermogenesis. (A) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with Pim/AKK1-IN-1 (1 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (B) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with STO-609 (10 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (C) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Camkkβ siRNA for 24 h and followed by HPF (5 μM) treatment for 24 h. (D) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated <t>with</t> <t>BAPTA-AM</t> (20 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (E) Ca 2+ signal upon HPF (20 μM) stimulation in C3H10T1/2-derived adipocytes transfected with Dlat siRNA ( n = 4). (F) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by STO-609 (10 μM) treatment for 24 h. (G) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h. (H) mRNA levels of the thermogenic genes in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h ( n = 3). (I) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by BAPTA-AM (20 μM) treatment for 24 h. (J) Representative images of JC-1 staining in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h, scale bar = 50 μm. (K) The ratio of JC-1 fluorescence intensity (green to red) in (J) ( n = 6). (L) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with vector or Dlat-Flag plasmids for 48 h, scale bar = 50 μm. (M) Quantification of (L). (N) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with the negative control or Dlat siRNA for 48 h, scale bar = 50 μm. (O) Quantification of (N). Data are presented as mean ± SEM, and tested for normality using Shapiro-Wilk normality test and Kolmogorov-Smirnov test. Significance was assessed by two-way ANOVA (E), or one-way ANOVA (H and K), or Student’s t test (M and O). * P < 0.05; ** P < 0.01; *** P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Bapta Am, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bapta+am/pmc04608792-215-12-16?v=Cayman+Chemical
Average 90 stars, based on 1 article reviews
bapta-am - by Bioz Stars, 2026-08
90/100 stars
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bapta-am  (Enzo Biochem)
90
Enzo Biochem bapta-am
HPF induces Dlat-mediated Ca 2+ release to activate Camkkβ-AMPK signaling and adipose thermogenesis. (A) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with Pim/AKK1-IN-1 (1 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (B) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with STO-609 (10 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (C) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Camkkβ siRNA for 24 h and followed by HPF (5 μM) treatment for 24 h. (D) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated <t>with</t> <t>BAPTA-AM</t> (20 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (E) Ca 2+ signal upon HPF (20 μM) stimulation in C3H10T1/2-derived adipocytes transfected with Dlat siRNA ( n = 4). (F) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by STO-609 (10 μM) treatment for 24 h. (G) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h. (H) mRNA levels of the thermogenic genes in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h ( n = 3). (I) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by BAPTA-AM (20 μM) treatment for 24 h. (J) Representative images of JC-1 staining in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h, scale bar = 50 μm. (K) The ratio of JC-1 fluorescence intensity (green to red) in (J) ( n = 6). (L) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with vector or Dlat-Flag plasmids for 48 h, scale bar = 50 μm. (M) Quantification of (L). (N) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with the negative control or Dlat siRNA for 48 h, scale bar = 50 μm. (O) Quantification of (N). Data are presented as mean ± SEM, and tested for normality using Shapiro-Wilk normality test and Kolmogorov-Smirnov test. Significance was assessed by two-way ANOVA (E), or one-way ANOVA (H and K), or Student’s t test (M and O). * P < 0.05; ** P < 0.01; *** P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Bapta Am, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bapta+am/10__1074_slash_jbc__m114__562900-59-0-6?v=Enzo+Biochem
Average 90 stars, based on 1 article reviews
bapta-am - by Bioz Stars, 2026-08
90/100 stars
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1,2-bis-(o-aminophenoxy)-ethane-n,n,n,n-tetraacetic acid tetraacetoxymethyl ester (bapta-am)  (Biomol GmbH)
90
Biomol GmbH 1,2-bis-(o-aminophenoxy)-ethane-n,n,n,n-tetraacetic acid tetraacetoxymethyl ester (bapta-am)
HPF induces Dlat-mediated Ca 2+ release to activate Camkkβ-AMPK signaling and adipose thermogenesis. (A) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with Pim/AKK1-IN-1 (1 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (B) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with STO-609 (10 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (C) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Camkkβ siRNA for 24 h and followed by HPF (5 μM) treatment for 24 h. (D) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated <t>with</t> <t>BAPTA-AM</t> (20 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (E) Ca 2+ signal upon HPF (20 μM) stimulation in C3H10T1/2-derived adipocytes transfected with Dlat siRNA ( n = 4). (F) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by STO-609 (10 μM) treatment for 24 h. (G) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h. (H) mRNA levels of the thermogenic genes in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h ( n = 3). (I) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by BAPTA-AM (20 μM) treatment for 24 h. (J) Representative images of JC-1 staining in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h, scale bar = 50 μm. (K) The ratio of JC-1 fluorescence intensity (green to red) in (J) ( n = 6). (L) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with vector or Dlat-Flag plasmids for 48 h, scale bar = 50 μm. (M) Quantification of (L). (N) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with the negative control or Dlat siRNA for 48 h, scale bar = 50 μm. (O) Quantification of (N). Data are presented as mean ± SEM, and tested for normality using Shapiro-Wilk normality test and Kolmogorov-Smirnov test. Significance was assessed by two-way ANOVA (E), or one-way ANOVA (H and K), or Student’s t test (M and O). * P < 0.05; ** P < 0.01; *** P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
1,2 Bis (O Aminophenoxy) Ethane N,N,N,N Tetraacetic Acid Tetraacetoxymethyl Ester (Bapta Am), supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bapta+am/10__1074_slash_jbc__m112__344309-55-0-9?v=Biomol+GmbH
Average 90 stars, based on 1 article reviews
1,2-bis-(o-aminophenoxy)-ethane-n,n,n,n-tetraacetic acid tetraacetoxymethyl ester (bapta-am) - by Bioz Stars, 2026-08
90/100 stars
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tf-bapta  (AG Scientific)
90
AG Scientific tf-bapta
HPF induces Dlat-mediated Ca 2+ release to activate Camkkβ-AMPK signaling and adipose thermogenesis. (A) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with Pim/AKK1-IN-1 (1 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (B) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with STO-609 (10 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (C) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Camkkβ siRNA for 24 h and followed by HPF (5 μM) treatment for 24 h. (D) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated <t>with</t> <t>BAPTA-AM</t> (20 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (E) Ca 2+ signal upon HPF (20 μM) stimulation in C3H10T1/2-derived adipocytes transfected with Dlat siRNA ( n = 4). (F) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by STO-609 (10 μM) treatment for 24 h. (G) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h. (H) mRNA levels of the thermogenic genes in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h ( n = 3). (I) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by BAPTA-AM (20 μM) treatment for 24 h. (J) Representative images of JC-1 staining in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h, scale bar = 50 μm. (K) The ratio of JC-1 fluorescence intensity (green to red) in (J) ( n = 6). (L) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with vector or Dlat-Flag plasmids for 48 h, scale bar = 50 μm. (M) Quantification of (L). (N) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with the negative control or Dlat siRNA for 48 h, scale bar = 50 μm. (O) Quantification of (N). Data are presented as mean ± SEM, and tested for normality using Shapiro-Wilk normality test and Kolmogorov-Smirnov test. Significance was assessed by two-way ANOVA (E), or one-way ANOVA (H and K), or Student’s t test (M and O). * P < 0.05; ** P < 0.01; *** P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Tf Bapta, supplied by AG Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bapta+am/pmc04304440__ja511313k_si_001-7-0-3?v=AG+Scientific
Average 90 stars, based on 1 article reviews
tf-bapta - by Bioz Stars, 2026-08
90/100 stars
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bis-(o-aminophenoxy)ethane-n,n,n9,n9-tetraacetic acid, tetra(acetoxymethyl) ester  (Biomol GmbH)
90
Biomol GmbH bis-(o-aminophenoxy)ethane-n,n,n9,n9-tetraacetic acid, tetra(acetoxymethyl) ester
HPF induces Dlat-mediated Ca 2+ release to activate Camkkβ-AMPK signaling and adipose thermogenesis. (A) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with Pim/AKK1-IN-1 (1 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (B) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with STO-609 (10 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (C) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Camkkβ siRNA for 24 h and followed by HPF (5 μM) treatment for 24 h. (D) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated <t>with</t> <t>BAPTA-AM</t> (20 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (E) Ca 2+ signal upon HPF (20 μM) stimulation in C3H10T1/2-derived adipocytes transfected with Dlat siRNA ( n = 4). (F) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by STO-609 (10 μM) treatment for 24 h. (G) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h. (H) mRNA levels of the thermogenic genes in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h ( n = 3). (I) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by BAPTA-AM (20 μM) treatment for 24 h. (J) Representative images of JC-1 staining in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h, scale bar = 50 μm. (K) The ratio of JC-1 fluorescence intensity (green to red) in (J) ( n = 6). (L) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with vector or Dlat-Flag plasmids for 48 h, scale bar = 50 μm. (M) Quantification of (L). (N) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with the negative control or Dlat siRNA for 48 h, scale bar = 50 μm. (O) Quantification of (N). Data are presented as mean ± SEM, and tested for normality using Shapiro-Wilk normality test and Kolmogorov-Smirnov test. Significance was assessed by two-way ANOVA (E), or one-way ANOVA (H and K), or Student’s t test (M and O). * P < 0.05; ** P < 0.01; *** P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Bis (O Aminophenoxy)ethane N,N,N9,N9 Tetraacetic Acid, Tetra(acetoxymethyl) Ester, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bapta+am/pm09169474-59-5-12?v=Biomol+GmbH
Average 90 stars, based on 1 article reviews
bis-(o-aminophenoxy)ethane-n,n,n9,n9-tetraacetic acid, tetra(acetoxymethyl) ester - by Bioz Stars, 2026-08
90/100 stars
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bapta-am  (AAT Bioquest)
90
AAT Bioquest bapta-am
HPF induces Dlat-mediated Ca 2+ release to activate Camkkβ-AMPK signaling and adipose thermogenesis. (A) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with Pim/AKK1-IN-1 (1 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (B) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with STO-609 (10 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (C) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Camkkβ siRNA for 24 h and followed by HPF (5 μM) treatment for 24 h. (D) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated <t>with</t> <t>BAPTA-AM</t> (20 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (E) Ca 2+ signal upon HPF (20 μM) stimulation in C3H10T1/2-derived adipocytes transfected with Dlat siRNA ( n = 4). (F) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by STO-609 (10 μM) treatment for 24 h. (G) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h. (H) mRNA levels of the thermogenic genes in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h ( n = 3). (I) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by BAPTA-AM (20 μM) treatment for 24 h. (J) Representative images of JC-1 staining in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h, scale bar = 50 μm. (K) The ratio of JC-1 fluorescence intensity (green to red) in (J) ( n = 6). (L) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with vector or Dlat-Flag plasmids for 48 h, scale bar = 50 μm. (M) Quantification of (L). (N) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with the negative control or Dlat siRNA for 48 h, scale bar = 50 μm. (O) Quantification of (N). Data are presented as mean ± SEM, and tested for normality using Shapiro-Wilk normality test and Kolmogorov-Smirnov test. Significance was assessed by two-way ANOVA (E), or one-way ANOVA (H and K), or Student’s t test (M and O). * P < 0.05; ** P < 0.01; *** P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Bapta Am, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bapta+am/pmc03711991-103-15-16?v=AAT+Bioquest
Average 90 stars, based on 1 article reviews
bapta-am - by Bioz Stars, 2026-08
90/100 stars
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bapta-am  (Biomol GmbH)
90
Biomol GmbH bapta-am
HPF induces Dlat-mediated Ca 2+ release to activate Camkkβ-AMPK signaling and adipose thermogenesis. (A) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with Pim/AKK1-IN-1 (1 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (B) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with STO-609 (10 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (C) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Camkkβ siRNA for 24 h and followed by HPF (5 μM) treatment for 24 h. (D) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated <t>with</t> <t>BAPTA-AM</t> (20 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (E) Ca 2+ signal upon HPF (20 μM) stimulation in C3H10T1/2-derived adipocytes transfected with Dlat siRNA ( n = 4). (F) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by STO-609 (10 μM) treatment for 24 h. (G) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h. (H) mRNA levels of the thermogenic genes in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h ( n = 3). (I) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by BAPTA-AM (20 μM) treatment for 24 h. (J) Representative images of JC-1 staining in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h, scale bar = 50 μm. (K) The ratio of JC-1 fluorescence intensity (green to red) in (J) ( n = 6). (L) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with vector or Dlat-Flag plasmids for 48 h, scale bar = 50 μm. (M) Quantification of (L). (N) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with the negative control or Dlat siRNA for 48 h, scale bar = 50 μm. (O) Quantification of (N). Data are presented as mean ± SEM, and tested for normality using Shapiro-Wilk normality test and Kolmogorov-Smirnov test. Significance was assessed by two-way ANOVA (E), or one-way ANOVA (H and K), or Student’s t test (M and O). * P < 0.05; ** P < 0.01; *** P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Bapta Am, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bapta+am/pm20101614-152-0-4?v=Biomol+GmbH
Average 90 stars, based on 1 article reviews
bapta-am - by Bioz Stars, 2026-08
90/100 stars
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HPF induces Dlat-mediated Ca 2+ release to activate Camkkβ-AMPK signaling and adipose thermogenesis. (A) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with Pim/AKK1-IN-1 (1 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (B) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with STO-609 (10 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (C) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Camkkβ siRNA for 24 h and followed by HPF (5 μM) treatment for 24 h. (D) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with BAPTA-AM (20 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (E) Ca 2+ signal upon HPF (20 μM) stimulation in C3H10T1/2-derived adipocytes transfected with Dlat siRNA ( n = 4). (F) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by STO-609 (10 μM) treatment for 24 h. (G) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h. (H) mRNA levels of the thermogenic genes in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h ( n = 3). (I) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by BAPTA-AM (20 μM) treatment for 24 h. (J) Representative images of JC-1 staining in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h, scale bar = 50 μm. (K) The ratio of JC-1 fluorescence intensity (green to red) in (J) ( n = 6). (L) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with vector or Dlat-Flag plasmids for 48 h, scale bar = 50 μm. (M) Quantification of (L). (N) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with the negative control or Dlat siRNA for 48 h, scale bar = 50 μm. (O) Quantification of (N). Data are presented as mean ± SEM, and tested for normality using Shapiro-Wilk normality test and Kolmogorov-Smirnov test. Significance was assessed by two-way ANOVA (E), or one-way ANOVA (H and K), or Student’s t test (M and O). * P < 0.05; ** P < 0.01; *** P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Journal: Journal of Advanced Research

Article Title: Targeting Dlat-Trpv3 pathway by hyperforin elicits non-canonical promotion of adipose thermogenesis as an effective anti-obesity strategy

doi: 10.1016/j.jare.2024.11.035

Figure Lengend Snippet: HPF induces Dlat-mediated Ca 2+ release to activate Camkkβ-AMPK signaling and adipose thermogenesis. (A) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with Pim/AKK1-IN-1 (1 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (B) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with STO-609 (10 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (C) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Camkkβ siRNA for 24 h and followed by HPF (5 μM) treatment for 24 h. (D) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes pretreated with BAPTA-AM (20 μM) or CC (10 μM) for 2 h and followed by HPF (5 μM) treatment for 24 h. (E) Ca 2+ signal upon HPF (20 μM) stimulation in C3H10T1/2-derived adipocytes transfected with Dlat siRNA ( n = 4). (F) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by STO-609 (10 μM) treatment for 24 h. (G) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h. (H) mRNA levels of the thermogenic genes in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h ( n = 3). (I) Immunoblots (protein indicated) in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids for 24 h and followed by BAPTA-AM (20 μM) treatment for 24 h. (J) Representative images of JC-1 staining in C3H10T1/2-derived adipocytes transfected with Dlat-Flag plasmids and Camkkβ siRNA for 48 h, scale bar = 50 μm. (K) The ratio of JC-1 fluorescence intensity (green to red) in (J) ( n = 6). (L) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with vector or Dlat-Flag plasmids for 48 h, scale bar = 50 μm. (M) Quantification of (L). (N) Representative images of Fluo-4 AM staining in C3H10T1/2-derived adipocytes transfected with the negative control or Dlat siRNA for 48 h, scale bar = 50 μm. (O) Quantification of (N). Data are presented as mean ± SEM, and tested for normality using Shapiro-Wilk normality test and Kolmogorov-Smirnov test. Significance was assessed by two-way ANOVA (E), or one-way ANOVA (H and K), or Student’s t test (M and O). * P < 0.05; ** P < 0.01; *** P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: For the compound treatment experiment, cells were incubated with 5 or 20 μM HPF (Sigma, H5160), or 10 μM L748337 (MedChemExpress, HY-103211), or 1 μM SR (MedChemExpress, HY-100672), or 1 μM Pim1/AKK1-IN-1 (MedChemExpress, HY-10371), or 0.05 μM Radicicol (Apexbio, A4067), or 10 μM STO-609 (Selleck, 8274), or 10 μM Compound C (MedChemExpress, HY-13418A), or 20 μM BAPTA-AM (Selleck, S7534) for the indicated times.

Techniques: Western Blot, Derivative Assay, Transfection, Staining, Fluorescence, Plasmid Preparation, Negative Control